Reference Sequence Metadata by MD5 Checksum: Length and Identifiers (GA4GH refget, ENA)
refget/sequence-metadata
Returns the GA4GH refget record for one reference sequence held in the European Nucleotide Archive's CRAM reference registry, looked up by its 32-character MD5 checksum: the sequence's length in bases, its MD5 id and any aliases, with the TRUNC512 and GA4GH digests when the registry has them (usually null). Use it to confirm a reference sequence exists and how long it is before fetching a window with refget/sequence-bases. Data from the European Nucleotide Archive (EMBL-EBI), served by Eden Engine; not an official ENA or EMBL-EBI product.
Price
$0.0026 per call
You pay the price shown. If a call turns out to cost more than its quote, you pay the actual price, capped at twice the quote. No monthly minimum. Top-ups start at $0.50.
Data source
European Nucleotide Archive (EMBL-EBI)
Data from the European Nucleotide Archive (ENA) CRAM reference registry, EMBL-EBI, through its GA4GH refget service. INSDC sequence data carries no use restrictions. Served by Eden Engine; not an official EMBL-EBI product.
Use it
Five steps: search, inspect, learn, run, settle. No per-tool setup; your agent reaches this tool through the meta-tools it already has.
Connect once and your AI can find and run this tool, and any other it needs.
// 1. search: find the tool
omnial_search({ query: "what you need" })
// 2. inspect: price and input schema
omnial_inspect({ tool: "refget/sequence-metadata" })
// 3. learn: read the usage guide at runtime
omnial_learn({ tool: "refget/sequence-metadata" })
// 4. run: price the exact call first, then run it
omnial_execute({ tool: "refget/sequence-metadata", input: { /* see input_schema */ }, dry_run: true })
omnial_execute({ tool: "refget/sequence-metadata", input: { /* ... */ } })
// 5. settle: the call is charged when it finishes, nothing to doNot connected yet? Point your MCP client at https://mcp.eden-engine.com/mcp, then copy a config for your client.
Pricing
Every charge this tool can incur.
| Charge | Price |
|---|---|
Per call | $0.0026 |
- Updated
- Oct 25, 2026
Input and output schemas
Input schema
{
"type": "object",
"required": [
"checksum"
],
"properties": {
"checksum": {
"type": "string",
"pattern": "^[0-9a-f]{32}$",
"description": "The MD5 checksum of the sequence as 32 lowercase hexadecimal characters, for example 3332ed720ac7eaa9b3655c06f6b9e196 (the phiX174 genome). Sequence accessions such as U00096.3 are not resolved by this service."
}
},
"additionalProperties": false
}Output schema
{
"type": "object",
"required": [
"id",
"md5",
"length",
"aliases"
],
"properties": {
"id": {
"type": "string",
"description": "The identifier the registry resolved; the MD5."
},
"md5": {
"type": "string",
"description": "MD5 checksum of the sequence."
},
"ga4gh": {
"type": [
"string",
"null"
],
"description": "GA4GH sequence identifier (sha512t24u), or null when not held."
},
"length": {
"type": "integer",
"description": "Sequence length in bases."
},
"aliases": {
"type": "array",
"items": {
"type": "object",
"additionalProperties": true
},
"description": "Other names the registry knows the sequence by; often empty."
},
"trunc512": {
"type": [
"string",
"null"
],
"description": "GA4GH TRUNC512 digest, or null when the registry does not hold it."
}
},
"description": "The registry's record for the sequence.",
"additionalProperties": true
}